Thp1 cell lines atcc

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August undefined, 2024

WebHEK293T (ATCC CRL-3216), L929 (ATCC CRL-6364), and J774 (ATCC TIB-67) were obtained from the American Type Culture Collection (ATCC). All of these cells were cultured in Dulbecco’s modified ... WebApr 30, 2024 · Human aortic endothelial cells (HAECs) were obtained from Lonza (Basel, Switzerland) and the human monocytic leukemia THP-1 cell line was purchased from …

Article - Human and Animal Health The Anti-inflammatory Effect …

Web3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at least twice the number of cells needed for the assay, because some cells will be lost throughout washes. In ADCP assays target cell number is usually the ... geforce unlocked

Cellosaurus cell line THP-1 (CVCL_0006)

WebJan 13, 2010 · The THP1 cell line was obtained from ATCC, and maintained at 2×10 5 cells/ml in RPMI 1640 medium supplemented with 10% FCS and 2 mmol/L L-glutamine. … WebIn THP1 cells, treatment with cGAMP ... T404 phosphorylation of STAT2 promotes resistance to cisplatin in lung cancer cell lines and inhibits IRF3 nuclear translocation in … WebCell lines Human THP-1 cells were obtained from ATCC and passaged in growth medium containing RPMI-1640 (Corning), ... Cells were pelleted and washed once more using the same buffer. Pellets were resuspended in 50ul of 0.5% SDS and incubates for 5-10 minutes at 62’C. Next reactions were quenched with 145ul of water and 25ul dc radar snowboard bag

IL-1 receptor–associated kinase-3 acts as an immune checkpoint …

THP-1 Cell Line - an overview ScienceDirect Topics

WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a temperature of 37 o C in a humidified incubator with … WebJul 9, 2015 · THP1 (ATCC ® TIB-202™) human monocyte cell lines were cultured in Roswell Park Memorial Institute medium 1640 containing 10% (v / v) fetal bovine serum, 0.05 mM … geforce unblockerWebApr 30, 2024 · Human aortic endothelial cells (HAECs) were obtained from Lonza (Basel, Switzerland) and the human monocytic leukemia THP-1 cell line was purchased from ATCC (USA). HAECs were maintained using a Microvascular Endothelial Cell Growth Medium-2 BulletKit from Lonza, USA. THP-1 cells were cultured in DMEM media containing 10 % … dcra drawing submission requirements

"WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase … " - Thp1 cell lines atcc

Thp1 cell lines atcc

Activation of GPR30 with G1 inhibits oscillatory shear stress …

WebAug 1, 2024 · THP-1 is a representative leukemia cell line and is registered with four different numbers in JCRB and RIKEN BRC cell banks. However, differences between … WebApr 13, 2024 · Two independent dataset with BRCA cell lines, CCLE breast cancer cell lines (n = 69) , and BRCA cell lines ... (0.8 µg/mL) was added in the media to maintain selection. …

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WebFirst, we analyzed whether TcpB suppresses intracellular LPS-induced pyroptotic cell death in a murine macrophage cell line (RAW264.7), primary bone marrow-derived … WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM …

WebApr 11, 2024 · Since we did not find GPR160 in PC12 cells, the only known cell line with specific binding of CARTp, and detected no specific binding on THP1 cells with high endogenous expression of GPR160 (www.proteinatlas.org), we transfected two human cell lines, U2OS and U-251 MG, both with low endogenous expression of, with GPR160 to … WebJan 13, 2024 · A THP-1 Cell Line-Based Exploration of Immune Responses Toward Heat-Treated BLG. Ying Deng 1,2, Coen Govers 1, Ellen ter Beest 1,2, Aalt-Jan van Dijk 3, Kasper Hettinga 4 and Harry J. Wichers 1,2*. 1 Food and Biobased Research, Wageningen University and Research, Wageningen, Netherlands. 2 Food Chemistry, Department of …

WebAfter exposure to PMA, the differentiated THP1 cells were treated with LPS, a bacterial endotoxin that has been used to trigger pro-inflammatory response in macrophage-like … WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research. Note: These are suspension cells and … See all our upcoming events focused on advancing global health with innovative … Genetic material deposited with ATCC after 12 October 2014 falls under the … ATCC is a nonprofit organization that collects, stores, and distributes standard …

WebCell Proliferation Assay. Cytotoxicity against the human leukemic monocyte cell line THP-1 and the embryonic fibroblast cell line BALB/3T3 was measured using a fluorometric …

WebApr 13, 2024 · Two independent dataset with BRCA cell lines, CCLE breast cancer cell lines (n = 69) , and BRCA cell lines ... (0.8 µg/mL) was added in the media to maintain selection. THP1 cells, also purchased from ATCC, were maintained and grown in RPMI-1640 medium with the above-mentioned supplements. 2.3. geforce update drivers 2021WebTHP-1 - Cell Line (ID:50861) HMS LINCS ID: 50861 Name: THP-1 Alternative Names: LINCS ID: LCL-1072 Alternative ID: CLO_0009348: Parent Cell Line: Reference Source: ATCC TIB-202: Organism: Homo sapiens Organ: blood Tissue: Cell Type: Details of Cell Type: Donor Sex: male Donor Age: Donor Ethnicity : Donor Health Status: Disease: AML Details of ... geforce unable to update settingsWebTo this end, we used the highly sensitive in situ proximity ligation assay 17 on the acute monocytic leukemia THP-1 cell line that is known to be responsive to both estrogen and … d craft events