WebAug 23, 2013 · The emerging theme that phospho-Ser/Thr-binding domains are master regulators of the cell cycle seems to have ancient origins, as Ser/Thr kinases and phospho-Ser/Thr-binding domains mediate cell ... WebThe Motif Logo was generated from a PhosphoScan ® LC-MS/MS experiment using 1794 nonredundant tryptic peptides derived from mitotic HeLa cells. HeLa cells were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and …
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WebPhospho- (Ser/Thr) Phe Antibody ELISAs: Signal to noise ratio of phospho- versus nonphospho-peptides in which the phosphorylation site is adjacent to phenylalanine, tyrosine or tryptophan. (T* and S* denote phosphorylated … WebApr 13, 2024 · In particular, we saw increased phosphorylation of connexin-43 at Ser325/Thr326, and decreased levels of the phospho-peptide, Ser-Thr 320-345 (Figure 4A’; Supplementary Table S4). Notably, the phosphorylation of Ser325 on connexin-43, along with nearby residues Ser328 and Ser330, is mediated by casein kinase 1 and has been shown … florist in philippi wv
Phospho-Ser/Thr-binding domains: navigating the cell …
Buffers and proteins which contain phosphate should be avoided with this antibody. Certain proteins known to contain phosphorylated serine and threonine may not be detected by this antibody due to steric hindrance. The immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE. WebMore>> Detect phospho-Ser/Thr-Pro also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, FITC conjugate (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC. Less<< MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS; CoA; WebAll lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution Lane 1 : Human THP-1 Whole Cell Extract Lane 2 : Mouse Total Lung Extract Lysates/proteins at 10 µg per lane. Secondary All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody Developed using the ECL technique. Performed under reducing conditions. great yarmouth circus opening times