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Peasy®-blunt zero cloning kit

WebThe Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of nonrecombinants. The - pCR®-Blunt vector …

拟南芥长链非编码RNA-DANA1及其在植物干旱耐受性的应用

WebUltra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases ... WebDetails. 6027. Mighty Cloning Reagent Set (Blunt End) 20 Rxns. USD $377.00. Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously, both blunt- and dA-ended products … top ent hospital in bangalore https://connersmachinery.com

A strategy for in-house production of a positive selection cloning ...

WebHigh cloning efficiency. Positive clones up to 100%. No blue/white selection needed. Suitable for short and large fragment cloning. Kanamycin and Ampicillin resistance genes … WebZero Blunt ® TOPO ® PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells containa box with Zero Blunt ® TOPO ® PCR Cloning reagents (Box 1) and a box with One Shot ® E. coli (Box 2). Zero Blunt ® TOPO ® PCR Cloning Kit supplied with the PureLink ® Quick Plasmid Miniprep Kit (Cat. no.K2800-02) is shipped with an ... WebZero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized … picture of a star cartoon

pEASY®-Blunt Simple Cloning Kit - CB111 Civic Bioscience

Category:Development of a reverse transcription recombinase-aided

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Peasy®-blunt zero cloning kit

Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO

WebPeasyblunt Zero Cloning Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article … WebpEASY® -T5 Zero Cloning Kit pEASY® -Blunt Zero Cloning Kit pEASY® -Uni Seamless Cloning and Assembly Kit pEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell

Peasy®-blunt zero cloning kit

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WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为:95 ℃预变性2 min;95 ℃ 30 s,55 ℃ 30 s,72 ℃ 1 min,30个循环;72 ℃ 延伸 2 min。PCR产物进行胶回收后,连接至pEASY-Blunt Zero Cloning Kit克隆载体,转化大肠杆菌感受态DH5α中,挑取单克隆测序。 WebApr 11, 2024 · The PCR program consisted of 3 min at 95°C, followed by 35 cycles of 15 s at 95°C, 15 s at 55°C, and 120 s at 72°C, followed by a 5 min extension at 72°C. The PCR product was inserted into the pEASY-Blunt Simple Cloning vector (TransGen Biotech, Beijing, China) and then transformed into DH5α for sequencing.

Web5 min TA/Blunt-Zero Cloning Kit C601. USD$ 201.00. ClonExpress II One Step Cloning Kit C112. USD$ 217.00. Ultra-Universal TOPO Cloning Kit C603. USD$ 196.00. Mut Express MultiS Fast Mutagenesis Kit V2 C215 ... Web7 rows · Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified ...

WebZero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized … WebpEASY-Blunt Simple (linearized) Sequence and Map Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a …

WebThe Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants. The pCR™-Blunt vector …

Web茶树磷酸烯醇式丙酮酸转运子csppt2基因的克隆和分析. 2024-04-22 纪志芳 甘玉迪 陈常颂 杨鼎俊 孙康 黎星辉 陈暄 top entertainment consulting firmsWebMay 10, 2024 · Cloning and expression of the C11orf96 protein The C11orf96 gene in the bait vector pEASY®-Blunt Zero Cloning Kit (abbreviated as pEBCK) was obtained by RT-PCR, and 1% agarose gel electrophoresis showed that the size of the cDNA fragment amplified by PCR was in the expected range. picture of a star in the skyWebFeatures of the pEASY®-T5 Zero Cloning Kit. 5 minutes fast ligation of Taq-amplified (3’A overhangs) PCR products. High cloning efficiency. Positive clones up to 100%. No … picture of a starbucks