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Hieffclone

Web20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, … Web翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科研客户数量多达1.2万家。

HieffClone®ZeroTOPO-TASimpleCloning Kit,MCS-Depleted

Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID fragment was amplified by PCR from a commercial gene (USA126049; Addgene, Watertown, MA, USA). The sequences of the primers were listed in Table S1. Web12 de nov. de 2024 · 一种pbiflu荧光标签表达载体的制备方法及其应用 技术领域 1.本发明属于分子生物学技术领域,具体涉及一种pbiflu荧光标签表达载体的制备方法及其应用。 背景技术: 2.黄色荧光蛋白(yellow fluorescent protein,yfp)是将绿色荧光蛋白(gfp)第203为苏氨酸突变体为色氨酸,可以稳定生色团在激发状态的偶极矩 ... fixer upper black shiplap wall https://connersmachinery.com

Frontiers Anti-Restriction Protein, KlcAHS, Promotes …

WebHieff Clone ® Plus One Step Cloning Kit 是一款简便、快速、高效的 DNA定向克隆产品,该试剂盒可以将PCR产物定向克隆至任何载体的任何位点, 可高效克隆 50 bp-10 kb片段。. 将载体线性化,并在插入片段正、反 … Web本试剂盒是利用拓扑异构酶 (Topoisomerase)高效快速连接DNA片段的原理进一步研发而成,与传统的T4连接酶相比,具有以下优势:1)快速,仅需 1-5 min即可完成连接反应。. … Web20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, E351A, V361A, and Q365A) were generated using Hieff Mut Site-Directed Mutagenesis Kit (YEASEN, 11003ES10). can mirage spawn in day

Upregulation of PAIP1 promotes the gallbladder tumorigenesis …

Category:The Establishment of Infectious Clone and Single Round Infectious ...

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Hieffclone

HieffClone零背_价格-品牌-详情介绍_丁香通 - biomart.cn

WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into … Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID …

Hieffclone

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WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB WebHieffClone®ZeroTOPO-BluntCloningKit零背景TOPO-Blunt平末端克隆试剂盒 10909ES20 20T 625.00 产品描述 本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA …

Web丁香通为您提供HieffClone™P商品详情介绍:价格:¥410,货号:10912ES,品牌:Yeasen翌圣,产地:上海,详见丁香通HieffClone™P商品详情页; 丁香通 丁香园 Web1 de dez. de 2024 · The enzymes using in molecular cloning experiment were Sap Ⅰ (NEB, USA), T4 DNA Ligase (Takara, Japan) and 2 × Hieffclone Enzyme Mix (YEASEN, …

Web6 de mar. de 2024 · Search worldwide, life-sciences literature Search. Advanced Search Web6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in the transformed E. coli by recombination according to HieffClone TM MultiOneStep Cloning kit (YEASEN, Shanghai, China).

WebRESEARCH ARTICLE T E of I C and S R I P for C A10 Min Wang1 • Jingjing Yan1 • Liuyao Zhu1 • Meng Wang1 • Lizhen Liu1 • Rui Yu1 • Ming Chen1 • Jingna Xun1 • Yuling Zhang1 • Zhigang Yi1 • Shuye Zhang1 Received: 27 September 2024/Accepted: 24 December 2024/Published online: 6 March 2024

WebYeasenBiotechnology(Shanghai)Co.,Ltd. E-mail:[email protected] www.yeasenbiotech.com 1 HB220923 HieffCloneTMZeroTOPO-TACloningKit ProductInformation Productname Cat# Size can miralax be given via feeding tubeWeb丁香通为您提供HieffClone™O商品详情介绍:价格:¥1 - 1680,货号:10905ES25,品牌:进口/国产,产地:德国/中国,详见丁香通HieffClone™O商品详情页; can miralax be given through a feeding tubeWebCN114134170A CN202411457529.2A CN202411457529A CN114134170A CN 114134170 A CN114134170 A CN 114134170A CN 202411457529 A CN202411457529 A CN 202411457529A CN 114134170 A CN114134170 A CN 114134170A Authority CN China Prior art keywords mcs product pgl primer nos Prior art date 2024-12-02 Legal status … can miralax be mixed with applesauceWeb丁香通为您提供HieffClone零背商品详情介绍:价格:¥530,货号:10907ES20,品牌:Yeasen翌圣,产地:上海,详见丁香通HieffClone零背商品详情页; can miralax be mixed with sodahttp://www.labbase.net/Supply/SupplyItems-4428942.html can miralax be mixed with spriteWeb翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科 … can miralax be given via peg tubeWebCN114107369A CN202411458461.XA CN202411458461A CN114107369A CN 114107369 A CN114107369 A CN 114107369A CN 202411458461 A CN202411458461 A CN 202411458461A CN 114107369 A CN114107369 A CN 114107369A Authority CN China Prior art keywords mcs pgl vector primer reaction Prior art date 2024-12-02 Legal status … fixer upper boot rack