Hieffclone
WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into … Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID …
Hieffclone
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WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB WebHieffClone®ZeroTOPO-BluntCloningKit零背景TOPO-Blunt平末端克隆试剂盒 10909ES20 20T 625.00 产品描述 本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA …
Web丁香通为您提供HieffClone™P商品详情介绍:价格:¥410,货号:10912ES,品牌:Yeasen翌圣,产地:上海,详见丁香通HieffClone™P商品详情页; 丁香通 丁香园 Web1 de dez. de 2024 · The enzymes using in molecular cloning experiment were Sap Ⅰ (NEB, USA), T4 DNA Ligase (Takara, Japan) and 2 × Hieffclone Enzyme Mix (YEASEN, …
Web6 de mar. de 2024 · Search worldwide, life-sciences literature Search. Advanced Search Web6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in the transformed E. coli by recombination according to HieffClone TM MultiOneStep Cloning kit (YEASEN, Shanghai, China).
WebRESEARCH ARTICLE T E of I C and S R I P for C A10 Min Wang1 • Jingjing Yan1 • Liuyao Zhu1 • Meng Wang1 • Lizhen Liu1 • Rui Yu1 • Ming Chen1 • Jingna Xun1 • Yuling Zhang1 • Zhigang Yi1 • Shuye Zhang1 Received: 27 September 2024/Accepted: 24 December 2024/Published online: 6 March 2024
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