WebDec 17, 2024 · Bacterial cytological profiling. At the 3 hour mark, samples were taken for fluorescence microscopy, similar to previously described with modifications 9,10,11.In brief, 8 µL cells were added to ... WebMar 27, 2024 · The establishment of methods to elucidate the mechanism of action (MOA) of A. baumannii-specific antibiotics is needed to develop novel antimicrobial therapeutics …
Bacterial Cytological Profiling (BCP) as a Rapid and Accurate ...
WebIn this study, we developed a high-resolution bacterial cytological profiling method that can capture morphological variations of bacteria upon antibiotic treatment. With an … WebSep 17, 2013 · Cytological profiling in eukaryotic cells relies upon measuring a large number of cytoskeletal markers . Because bacteria are 100 times smaller than the typical … classroom secrets year 3 addition
Bacterial Cytological Profiling as a Tool To Study Mechanisms
Webthe multiplex cytological profiling protocol, images were captured at 20x magnification with an automated epifluorescent microscope. We extracted 824 morphological features (Table S2) from each cell using the open-source software CellProfiler [6]. A number of cellular phenotypes could be detected by eye (Figure 1B). WebSep 18, 2024 · Screening ecosystems containing at least one antiviral sample cytological profile were cross-referenced to cytological profiles obtained by the same method from a library of 720 bioactive reference compounds (Bray et al. 2024; Pennisi 2016). Orthogonal analyses using cytological profiling revealed HIV-inhibitory MeE samples in four major … WebSep 17, 2013 · A powerful emerging technique based on cytological profiling has been successfully used to identify the cellular pathways targeted by the inhibitors (Nonejuie et … classroom secrets year 3 fractions