WebIsolation of resident macrophages from mouse tissues involves complex procedures for a small yield. This is inconvenient for many functional macrophage assays, which require … WebDec 1, 2008 · In this protocol, bone marrow cells are grown in culture dishes in the presence of M-CSF, which is secreted by L929 cells and is used in the form of L929 …
A comprehensive guide to studying inflammasome activation and ... - Nature
WebMay 15, 2024 · Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). ... Following this protocol, you should obtain around 60 × 10 6 bone marrow cells per mouse, giving rise to 30 × 10 6 BMDMs. 6. This … WebThis chapter outlines protocols used to isolate and culture murine bone marrow-derived and peritoneal macrophages. Furthermore, we describe how these macrophages can be "polarized" to obtain specific macrophage subsets with special relevance to atherosclerosis. Keywords: Atherosclerosis; Bone marrow-derived macrophages; Macrophage … how much sugar is in tea
Isolation and culture of murine bone marrow-derived
WebJan 1, 2013 · 1. Phosphate-buffered saline (PBS). 2. 70 % Ethanol. 3. Roswell Park Memorial Institute media (RPMI)-1640. 4. Complete RPMI: 500 ml of RPMI-1640, 5.5 × 10 5 mol/l of 2-mercaptoethanol, 25 mM/l of HEPES, 100 U/ml of penicillin, 100 μg/ml of streptomycin sulfate, 50 ml of heat-inactivated fetal bovine serum (use serum of sufficient … WebMar 19, 2024 · Differentiation into bone marrow-derived macrophages. 15. ... The BMDM cultures described in this protocol, ... loss. Additionally, some mouse models may have … WebFeb 25, 2024 · In this chapter, we describe a protocol for the isolation of bone marrow cells from a mouse and their subsequent differentiation into BMDMs. The differentiation … men\u0027s black watch shirt